| Catalog Number | GTX30764 |
| Product Name | ACC1 (phospho Ser79) antibody |
| Product Description | Rabbit polyclonal to ACC1 (phospho ser79) |
| Synonyms | Acetyl Coenzyme A Carboxylase ACAC antibody ACC alpha antibody ACC beta antibody ACC1 antibody ACC2 antibody ACCA antibody ACCB antibody Acetyl CoA carboxylase 1 antibody Acetyl CoA carboxylase alpha antibody Biotin carboxylase antibody COA1 antibody COA2 antibody HFA1 antibody |
| Background | In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation. Activities of ACC-1 and 2 are regulated by their phosphorylation by 5-AMP-activated protein kinase (AMPK). Diabetes deranges AMPK master-switch and represses the ACC-1 gene-expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. ACC1 is also known as ACC-alpha is a cytosolic enzyme, enriched in liver, adipose and lactating mammary tissues. ACC1 catalyzes the carboxylation of acetyl-CoA to form malonyl-CoA, the rate-limiting step in the biogenesis of LCFA-CoA. ACC1 carries three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase (catalytic activity). Two variants of ACC-1 have been described. One with 8 additional amino acids commencing at Pro-1196. The other which is 59aa shorter than the predominant fat and liver isoform exist in mammals. The presence of 8 additional amino acids inhibits the in vitro phosphorylation of the Ser1200 by camp-dependent kinase. The two ACC1 isoforms are differentially regulated in a tissue specific manner and under different physiological conditions. The activity of ACC1 is finely regulated by hormone dependent phosphorylation and dephosphorylation. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Immunogen | Synthetic peptide - amino acids 73?85 of rat AcCoA carboxylase [C-HMRSSM[pS]GLHLVK] (KLH) |
| Antigen Species | Rat |
| Tested Applications | Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For WB: Use at a concentration of 0.5-2ug/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | AcCoA Carboxylase phosphorylated at Ser79 |
| Species Cross Reactivity | Human, Mouse, Rat and Rabbit - Not yet tested in other species |
| Positive Controls | RIPA lysate from mouse heart cytosol |
| Target | ACC1 (phospho Ser79) |
| Form Supplied | Liquid |
| Purification | Protein A affinity purified |
| Storage Buffer | PBS pH7.4 containing 0.05% sodium azide and 30% glycerol |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
