Catalog Number | GTX26188 |
Product Name | M13 antibody |
Product Description | Rabbit polyclonal to M13 Bacteriophage Coat Proteins |
Background | May act as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage. May be used as a reagent in ""phage ELISA"" offering sensitive and specific activity for detection of recombinant phages. |
Clonality | Polyclonal |
Host | Rabbit |
Isotype | IgG |
Immunogen | fd bacteriophage |
Tested Applications | ELISA, Indirect ELISA, Immunofluorescence. The usefulness of this product in other applications has not been determined. |
Application Note | IF: Use at an assay dependant dilution. I-ELISA: at least 1/1000 for 5 x10^7 phage/mlat least 1/8000 for 5 x 10^10 phage/ml Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
Specificity | Binds specifically to phage coat proteins of M13 or fd phage. |
Target | M13 |
Form Supplied | Liquid - IgG fraction of rabbit antiserum, in PBS with sodium azide |
Purification | IgG fraction |
Storage Buffer | 0.01M PBS pH7.4, 15mM Na azide |
Storage Instruction | Store at -20 ¡ÆC |
Notes | Concentration: 5.5 mg/ml- 7.5 mg/ml |