| Catalog Number | GTX11337 |
| Product Name | l Afadin antibody |
| Product Description | Rabbit polyclonal to l Afadin |
| Background | Afadin is a cell-cell adherens junction F-actin binding multidomain protein. Two splice variants of afadin have been described: l-afadin, which is ubiquitously expressed, and the smaller s-afadin, which is abundantly expressed in neural tissue. l-Afadin contains one PDZ domain near its middle followed by three proline-rich and one F-actin-binding region at the carboxyl-terminal. l/s-Afadin is localized to cell-cell junctions and binds several cytoplasmic proteins such as the small GTPase Ras, the tight junction protein ZO-1, and the vinculin binding protein ponsin/SH3P12. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Immunogen | Synthetic peptide conjugated to maleimide-activated KLH, corresponding to amino acids 1814-1829 of Rat I Afadin with N-terminal cysteine. |
| Antigen Species | Rat |
| Tested Applications | Immunofluorescence, Immunohistochemistry, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | IF: Use at a dilution of 1/500 - 1/1000. IHC: Use at an assay dependent dilution. WB: Use at a dilution of 1/2000. Detects a band of approximately 200 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Species Cross Reactivity | Cross-reacts with Human, Mouse, Rat and Dog. Not yet tested in other species. |
| Target | l Afadin |
| Purification | Immunogen affinity purified |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
| Storage Instruction | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Notes | Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours." |
