| Catalog Number | GTX30456 |
| Product Name | Acid-Sensing Ionic Channel Beta Antibody (ASIC) |
| Product Description | Polyclonal Anti - Beta ASIC |
| Background | Two proton-gated channels, acid-sensing ionic channel (ASIC) and dorsal root ASIC (DRASIC), are known to be expressed by sensory neurons. These types of channels are of interest because low pH causes pain. ASIC Beta, a splice variant of ASIC, is found only in a subset of small and large diameter sensory neurons and is absent from sympathetic neurons or the central nervous system.. |
| Clonality | Polyclonal |
| Host | Guinea Pig |
| Immunogen | Residues 3-18 of rat ASIC Beta. AGSELDEGDDSPRDLV |
| Antigen Species | Rat |
| Tested Applications | Immunohistochemistry. The usefulness of this product in other applications has not been determined. |
| Application Note | Immunohistochemistry 1:1,000 |
| Specificity | Reacts with rat ASIC Beta. |
| Species Cross Reactivity | This antibody reacts with rat ASIC Beta. Other species have not been tested. |
| Positive Controls | Rat spinal cord. Staining was blocked by preabsorption with the cognate peptide. |
| Target | Beta ASIC |
| Form Supplied | Liquid |
| Purification | Whole Antiserum |
| Storage Buffer | antisera in 0.05% sodium azide |
| Storage Instruction | Store at ?20C. Avoid repeated freeze-thaw cycles. |
| Notes | Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4?C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. |
