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Cat. No |
Model |
Description |
Unit |
Price (VATº°µµ) |
³³±â |
Àç°í |
DataSheet |
ÁÖ¹®/°ßÀû |
LCA-25-394597 |
GTX72626(R) |
Actin [1A4] antibody (TCS)//Human,Rabbit,Baboon,Mouse,Bovine,Rat,Chicken/Mouse |
1/EA |
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Subject
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Actin [1A4] antibody (TCS) |
Description
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/ Human,Rabbit,Baboon,Mouse,Bovine,Rat,Chicken/Mouse |
Clonality
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Mono |
Company
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GeneTex |
Application
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IF, IHC-Fr, IHC-P |
Conjugation
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Immunogen
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Contents
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Catalog Number | GTX72626 | Product Name | Actin [1A4] antibody (TCS) | Product Description | Mouse monoclonal [1A4] to Actin (TCS) | Background | Smooth muscle actin is expressed in normal smooth muscle and in myoepithelial cells, and also in tumours derived from these cells. Actins are involved in maintaining the structure and integrity of a cell, as well as cell motility. They are highly conserved proteins and make up the majority of the contractile apparatus. To date, six different actin isoforms have been described. | Clonality | Monoclonal | Clone No | 1A4 | Host | Mouse | Isotype | IgG2a | Immunogen | The N-terminal decapeptide of alpha-smooth muscle actin. | Tested Applications | Immunofluorescence, IHC (Frozen sections), IHC (Formalin-fixed paraffin-embedded sections). The usefulness of this product in other applications has not been determined. | Application Note | For IHC-P: Use at a dilution of 1:50 - 1:75. For IHC-Fr: Use at a dilution of 1:50 - 1:75. Prolonged fixation in buffered formalin can destroy the epitope. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. | Specificity | This antibody reacts with the alpha-smooth muscle isoform of actin. It does not react with actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial). | Species Cross Reactivity | Human, Rabbit, Baboon, Mouse, Bovine, Rat and Chicken - Not yet tested in other species | Positive Controls | Bowel | Target | Actin Alpha-Smooth Muscle | Cellular Localization | Cytoplasmic | Form Supplied | Liquid | Purification | Unpurified | Purification Note | From hybridoma culture supernatant | Storage Buffer | 20 mM tris-borate, 150 mM sodium chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovineserum, BMC-6 carrier polysaccharides, carrier protein, and 0.1% sodium azide, pH 7.5. | Storage Instruction | Keep as concentrated solution, aliquot and store at 4¡ÆC. Do not freeze. | |
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