| Catalog Number | GTX73664 |
| Product Name | Actin muscle [HHF35] antibody |
| Product Description | Monoclonal Mouse Antibody to Human Muscle Actin |
| Background | This antibody is specific to alpha and gamma specific actin isomers from skeletal, cardiac and smooth muscle but does not recognize beta and nonsmooth muscle gamma actin isomers. In Western blot, HHF35 picks up 42 kD protein extract from skeletal muscle but not from epithelial cell line. |
| Clonality | Monoclonal |
| Clone No | HHF35 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | SDS extracted protein from human myocardium. |
| Antigen Species | Human |
| Tested Applications | The usefulness of this product in other applications has not been determined. |
| Application Note | This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. |
| Positive Controls | Skeletal muscle |
| Target | Actin muscle |
| Cellular Localization | Cytoplasmic |
| Form Supplied | Liquid |
| Purification | This antibody is supplied as tissue culture supernatant containing sodium azide as preservative. |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. |
