| Catalog Number | GTX96190 |
| Product Name | ACTN1 antibody |
| Product Description | Mouse polyclonal to ACTN1 |
| Background | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. |
| Clonality | Polyclonal |
| Host | Mouse |
| Immunogen | ACTN1 (NP_001093, 543 a.a. ~ 640 a.a) partial recombinant protein with GST tag. |
| Antigen Species | Human |
| Tested Applications | ELISA, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For WB: (tested against MES-SA/Dx5 lysate): Use at an assay dependednt dilution. For ELISA (tested with immunogen): Use at an assay dependednt dilution. For WB: (tested against immunogen): Use at an assay dependednt dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
| Specificity | Not tested |
| Species Cross Reactivity | Human - Not yet tested in other species |
| Positive Controls | Recombinant protein |
| Target | ACTN1 |
| Form Supplied | Liquid |
| Purification | Unpurified |
| Purification Note | From polyclonal serum |
| Storage Buffer | Antiserum containing 50% glycerol |
| Storage Instruction | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Notes | Antibody reactive against recombinant protein with GST tag on ELISA. Please enquire about availability of recombinant protein. |
| Manufacturer & Mfg Part# | Abnova H00000087-A01Only available to US end-users. Not available outside of the US or for resale inside of the US. |
