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Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-25-394644 |
GTX11568 |
ADAMTS5 antibody//Cross/Rabbit |
1/EA |
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문의 |
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Subject
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ADAMTS5 antibody |
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Description
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/ Cross/Rabbit |
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Clonality
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Mono |
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Company
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GeneTex |
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Application
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Western Blot |
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Conjugation
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Immunogen
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Contents
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| Catalog Number | GTX11568 | | Product Name | ADAMTS5 antibody | | Product Description | Rabbit polyclonal to ADAMTS5 | | Synonyms | A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 ADMP2 Aggrecanase 2 Implantin | | Background | ADAMTS5, also known as Implantin or Aggrecanase 2, is a member of the larger family of ADAMs (A Disintegrin And Metalloproteinase) metalloproteinases containing thrombospondin (TS) repeats. ADAMTS5 (A Disintegrin And Metalloproteinase with Thrombospondin-5 motif) was first described as ADAMTS5, a protein elevated in mice during the peri-implantion period. At the same time, another group identified Aggrecanase 11, a protein elevated in arthritic synovium. The name was later changed to ADAMTS5. ADAMTS5 is expressed in human and mouse. It has been found in heart, lung, cervix, uterus, ovary, brain, cartilage, and numerous other tissues, as well as chondroblastoma cell lines. Initial observations indicated a role for ADAMTS5 in aggrecan cleavage and cartilage destruction, especially in arthritis and potentially a role in embryo implantion. ADAMTS5 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to efficiently cleave Aggrecan. In addition to the metalloprotease domain, ADAMTS5 has a propeptide domain, a prohormone convertase (PC, furin) cleavage site, a cysteine-rich domain, a spacer domain, and two thrombospondin-1 like domains. ADAMTS5 is inhibited by the endogenous MMP inhibitors (TIMP1, 2, 3, and 4) but most efficiently by TIMP3. Unlike many of the ADAMs proteases, ADAMTS5 does not have a transmembrane domain, and is a secreted protein. Full length ADAMTS5 is a 930 amino acid protein with a predicted molecular mass is 101.7 kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS5 a greater apparent molecular weight on reduced SDS PAGE gels. When ADAMTS5 is secreted, it is cleaved at the furin cleavage site (predicted molecular mass 73.2 kDa) and then further cleaved to generate a range of smaller forms. | | Clonality | polyclonal | | Host | Rabbit | | Isotype | IgG | | Immunogen | Synthetic peptide corresponding to the C-terminal (Human). | | Antigen Species | Human | | Tested Applications | Western blot. The usefulness of this product in other applications has not been determined. | | Application Note | WB: Use at a dilution of 1/1000. A starting antibody dilution of 1/5000 is recommended for chemiluminescent substrates. Higher antibody dilutions may be necessary for non-human samples. Detects, under reducing conditions, the zymogen form at 120 kDa, activated form at 73 kDa (major band), and breakdown products at 50 kDa and 40 kDa in cell lysates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. | | Species Cross Reactivity | Cross-reacts with Human. Not yet tested in other species. | | Target | ADAMTS5 | | Cellular Localization | Secreted. Associated with the extracellular matrix. | | Purification | Immunogen affinity purified | | Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS with 50% Glycerol | | Notes | If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use." | |
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