| Catalog Number | GTX76190 |
| Product Name | 1A antigen [MRC OX-6] antibody |
| Product Description | Mouse monoclonal [MRC OX-6] to RT1B |
| Synonyms | RT1 class Ia, locus A1 RT1A-1; RT1.A1(N) |
| Background | RT1A-1 may be involved in antigen presentation. It also serves as a ligand for the Ly-49W activating receptor and the Ly-49G(BALB/c) inhibitory receptor. |
| Clonality | Monoclonal |
| Clone No | MRC OX-6 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Rat Thymocyte membrane glycoprotein |
| Antigen Species | Rat |
| Tested Applications | Flow cytometry/FACS, IHC (Frozen sections), IHC (Formalin-fixed paraffin-embedded sections), Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For Flow cytometry and IHC-P: Use at a dilution of 1:50 - 1:100. For IHC-Fr and WB: Use at an assay dependent dilution. Not tested in other applications. Optimal concentrations/dilutions should be determined by the researcher. |
| Specificity | Recognizes Ia from all rat strains tested. It labels a monomorphic determinant of the rat Ia antigen present on the majority of peripheral B lymphocytes, dendritic cells, some macrophages, and certain epithelial cells but not peripheral T lymphocytes. This antibody also cross reacts with murine Ia antigens (particularly with k and s haplotypes). Analysis of recombinant strains shows that the determinant maps to the I-A region of the mouse MHC and correlates with mouse Ia specificity 17 or 18. Thus, the determinant recognized is polymorphic in the mouse. This antibody may be useful for recognizing the rat Ia antigen and can be coupled to Sepharose 4B for purification of rat Ia antigen by antibody affinity chromatography. |
| Species Cross Reactivity | Mouse - Not yet tested in other species |
| Target | 1A antigen |
| Form Supplied | Liquid |
| Purification | Protein G affinity purified |
| Purification Note | From hybridoma culture supernatant |
| Storage Buffer | Phosphate buffered saline, pH 7.2, containing 0.09% sodium azide |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
Image:img22247.gifStaining of rat spleen cells with mouse anti rat RT1B (GTX76190). Print:Print
