| Catalog Number | GTX76189 |
| Product Name | 1A antigen [MRC OX-6] antibody (FITC) |
| Product Description | Mouse monoclonal [MRC OX-6] to RT1B (FITC) |
| Background | Recognises a monomorphic determinant of the rat Ia antigen, present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. The antibody cross-reacts with certain strains of mice of MHC haplotypes k and s. |
| Clonality | Monoclonal |
| Clone No | MRC OX-6 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Rat thymocyte membrane glycoprotein |
| Antigen Species | Rat |
| Tested Applications | Flow cytometry/FACS. The usefulness of this product in other applications has not been determined. |
| Application Note | For FACS: Use at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | Recognizes Ia from all rat strains tested. It labels a monomorphic determinant of the rat Ia antigen present on the majority of peripheral B lymphocytes, dendritic cells, some macrophages, and certain epithelial cells but not peripheral T lymphocytes. This antibody also cross reacts with murine Ia antigens (particularly with k and s haplotypes). Analysis of recombinant strains shows that the determinant maps to the I-A region of the mouse MHC and correlates with mouse Ia specificity 17 or 18. Thus, the determinant recognized is polymorphic in the mouse. This antibody may be useful for recognizing the rat Ia antigen and can be coupled to Sepharose 4B for purification of rat Ia antigen by antibody affinity chromatography. |
| Species Cross Reactivity | Rat and Mouse - Not yet tested in other species |
| Target | 1A antigen |
| Conjugation | FITC |
| Form Supplied | Liquid |
| Purification | Protein G affinity purified |
| Purification Note | From mouse ascitic fluid |
| Storage Buffer | PBS, pH7.2 containing BSA and 0.02% sodium azide |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
