| Catalog Number | GTX17923 |
| Product Name | 160kDa Neurofilament medium [SPM202] antibody (ready-to-use) |
| Product Description | Mouse monoclonal [SPM202] to 160 kD Neurofilament Medium, prediluted |
| Synonyms | 4741 | NEF3 | 162250 | P07197 | NEFM | NF-M | NFM | |
| Background | Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to NF-M are useful to identify this protein and identify neurons and their processes in tissue sections and in tissue culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrigs disease or Alzheimers disease. |
| Clonality | Monoclonal |
| Clone No | SPM202 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Full length native protein (purified) (Human). |
| Antigen Species | Human |
| Tested Applications | IHC (Formalin-fixed paraffin-embedded sections). The usefulness of this product in other applications has not been determined. |
| Application Note | IHC-P: Use at prediluted concentration. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Species Cross Reactivity | Human - Not yet tested in other species |
| Positive Controls | IMR-5 cells Brain |
| Target | 160kDa Neurofilament medium |
| Cellular Localization | Cytoplasmic |
| Form Supplied | Liquid - Prediluted |
| Purification | Protein G purified |
| Storage Buffer | PBS, pH 7.4 containing 1%BSA and 0.1% sodium azide |
| Storage Instruction | Keep as concentrated solution and store at 4°C. Do not freeze. |
