| Catalog Number | GTX17879 |
| Product Name | 200kDa Neurofilament heavy [SPM203] antibody |
| Product Description | Mouse monoclonal [SPM203] to 200 kD Neurofilament Heavy |
| Synonyms | 4744 | NEFH | 105400 | P12036 | NFH | |
| Background | Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appear as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins known as NFL, NFM and NFH. Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable. |
| Clonality | Monoclonal |
| Clone No | SPM203 |
| Host | Mouse |
| Isotype | IgM |
| Immunogen | Full length native protein (purified from brain) (Human). |
| Antigen Species | Human |
| Tested Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | IHC-P: 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. WB: Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 200 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Species Cross Reactivity | Human - Not yet tested in other species |
| Positive Controls | IMR-5 cells. Brain. |
| Target | 200kDa Neurofilament heavy |
| Form Supplied | Liquid |
| Purification | IgG fraction |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
Image:img30497.jpgImmunohistochemistry using GTX17879 on human cerebellum counterstained with haemotoxylin Print:Print
