Catalog Number | GTX73804 |
Product Name | ZAP-70 Antibody |
Product Description | Monoclonal Mouse Antibody to ZAP - 70 |
Background | Mutations in ZAP-70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP-70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course. Recent studies suggest that protein levels of ZAP-70 are elevated in B-cells of CLL patients with non-mutant heavy-chain variable region (IgVH) but not those with the mutant regions. Recent evidence suggests that ZAP-70 could be an excellent prognostic biomarker with high levels of the proteins indicating a poor prognosis. |
Clonality | Monoclonal |
Clone No | 2F3.2 |
Host | Mouse |
Isotype | IgG2a |
Immunogen | GST-fusion to tandem SH2 domains of human ZAP-70 corresponding to residues 1-254. |
Antigen Species | Human |
Tested Applications | IHC (Formalin-fixed paraffin-embedded sections). The usefulness of this product in other applications has not been determined. |
Application Note | This antibody may be diluted to a titer of 1:50-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. |
Positive Controls | Tonsil |
Target | ZAP-70 |
Cellular Localization | Cytoplasmic |
Form Supplied | Liquid |
Purification | This antibody is supplied as liquid containing sodium azide as preservative. |
Storage Instruction | Store the antibody at 2-8 ¡ÆC. |