| Catalog Number | GTX30625 |
| Product Name | 15-PGDH antibody |
| Product Description | Rabbit polyclonal antibody to 15 PGDH |
| Synonyms | 3248 | HPGD | 601688 | P15428 | 15-PGDH | PGDH | PGDH1 | |
| Background | 15-Hydroxyprostaglandin dehydrogenase (15-PGDH) is a novel tumor suppressor in the COX-2 pathway. The protein is found in many normal tissues and appears to be down-regulated in colorectal and lung carcinoma tissue. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Immunogen | Purified type I human placental 15-PGDH protein. |
| Antigen Species | Human |
| Tested Applications | Immunofluorescence, IHC (Formalin-fixed paraffin-embedded sections), Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | This antibody may be used for Western blotting. Past lots have been used for immunohistochemistry (paraffin-embedded sections) and immunofluorescence but this lot has not been tested in such applications. In Western blot bands may be seen at 29 kDa (the active form) and 58 kDa. Suggested working dilutions: * Western Blot: 1:1,500-1:10,000 Immunofluorescence: (+) Immunohistochemistry: (+) *The investigator should determine the optimal working dilution for a specific application. |
| Specificity | This antibody is specific for 15-PGDH. |
| Species Cross Reactivity | This antibody reacts with human 15-PGDH protein. No other species have been tested. |
| Positive Controls | Treated A549 lung carcinoma cell lysates and LoVo colon carcinoma cell lysates. |
| Target | 15-PGDH |
| Cellular Localization | Cytoplasmic |
| Form Supplied | 0.1 ml of protein A purified rabbit sera. |
| Storage Buffer | Tris-glycine, 150mM NaCl, 0.05% sodium azide |
| Storage Instruction | Store at 48C. For long-term storage, aliquot into working volumes and store at -20 degrees Celcius. Avoid freeze-thaw cycles. |
| Notes | A549 cell lysates were treated: IL-6, DHT, and forskolin " |
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