| Catalog Number | GTX13047 |
| Product Name | Rab3C antibody |
| Product Description | Rabbit polyclonal to Rab3C |
| Synonyms | 171058 | Rab3c | |
| Background | Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins: synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in co-ordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 Rab proteins that have been described in mammals. Rab 3C is found in brain, adrenal gland and pituitary. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Immunogen | Synthetic peptide: MASAQDARFGQKDSSDQN (with additional C-terminal cycteine) conjugated to KLH, corresponding to amino acids 9-26 of Rat Rab3C. |
| Antigen Species | Rat |
| Tested Applications | Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | WB: Use at a concentration of 2 μg/ml. Predicted molecular weight: 22 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | Anti-Rab 3C recognizes Rab 3C (22 kDa) in immunoblots of rat brain and total cell protein extracts of the mouse cell line AtT20. |
| Species Cross Reactivity | Cross-reacts with Mouse and Rat. Expected to cross-react with Human (78% identity with immunogen) and Cow (94% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Target | Rab3C |
| Purification | Protein A purified |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 1mg/ml BSA |
| Storage Instruction | S |
| Notes | If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours." |
