| Catalog Number | GTX73051 |
| Product Name | p16 INK4a Antibody |
| Product Description | Rabbit Polyclonal to p16 INK4a |
| Background | p16INK4a is a tumor suppressor protein. It is a specific inhibitor of cdk4/cdk6, and a tumor suppressor involved in the pathogenesis of a variety of malignancies. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Immunogen | A synthetic peptide, corresponding to aa133-146 (Cys-NHARIDAAEGPSDI)from the C-terminus of human p16 ink4a |
| Antigen Species | Human |
| Tested Applications | Functional studies, IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation. The usefulness of this product in other applications has not been determined. |
| Application Note | Kinase Assay; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. This antibody co-precipitates cdk4 and cdk6 along with p16 INK4a. It also supports the activity of cdk4 and cdk6 enzymes. |
| Species Cross Reactivity | Human. Does not react with Mouse and Rat. Others not tested. |
| Positive Controls | HeLa Cells, Colon Carcinoma |
| Target | p16INK4a |
| Form Supplied | Liquid |
| Purification | Protein A affinity purified |
| Purification Note | Unpurified polyclonal serum |
| Storage Buffer | Prepared at 1mg/ml in 10mM PBS, pH 7.4, with 0.2% BSA & 0.09% sodium azide. |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
