| Catalog Number | GTX73879 |
| Product Name | P16 INK4a protein [JC8] Antibody |
| Product Description | Monoclonal Mouse Antibody to Human P16INK4a protein |
| Background | p16 is a mitotic inhibitor protein. It competes with D-type cyclins to bind to cdk4 and cdk6. It acts as tumor suppressor and inhibits the progression of cells through the G1 phase of the cell cycle. |
| Clonality | Monoclonal |
| Clone No | JC8 |
| Host | Mouse |
| Isotype | IgG2a |
| Immunogen | Purified human recombinant prokaryotic full length human p16 protein. |
| Antigen Species | Human |
| Tested Applications | IHC (Formalin-fixed paraffin-embedded sections). The usefulness of this product in other applications has not been determined. |
| Application Note | This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the use. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. |
| Positive Controls | Colan carcinoma |
| Target | P16INK4a protein |
| Cellular Localization | Nuclear and cytoplasmic |
| Form Supplied | Liquid |
| Purification | This antibody is supplied as purified immunoglobulin containing sodium azide as preservative. |
| Storage Instruction | Store the antibody at 4-8 °C. |
Image:GTX73879_Mob213.jpgp16INK4a?Formalin fixed paraffin embedded human colon carcinoma stained with p16 (cat. # GTX73879) using ABC and AEC chromogen.Print:Print
