Catalog Number | GTX30790 |
Product Name | p19 skp1 (S-phase Kinase-associated Protein) antibody |
Product Description | Mouse monoclonal [3G222] to p19 skp1 |
Synonyms | cyclin A/CDK2-associated p19 EMC19 OCP-II OCP2 organ of Corti protein 2 p19A p19skp1 RNA polymerase II elongation factor-like protein OCP2 SIII TCEB1L Transcription Elongation Factor B 1-like |
Background | Skp1 is an F-box protein which functions as a substrate recognition component of the SCF ubiquitin ligase complex. It binds to cyclin F, S-phase kinase-associated protein 2, and other regulatory proteins involved in ubiquitin proteolysis through an F-box motif. It is also involved in the control of beta-catenin levels the activity of beta-catenin dependent TCF transcription factors. Studies have also characterized the protein as an RNA polymerase II elongation factor. |
Clonality | Monoclonal |
Clone No | 3G222 |
Host | Mouse |
Isotype | IgG1 |
Immunogen | Recombinant human SKP1 (p19) protein |
Antigen Species | Human |
Tested Applications | ELISA, Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | For ELISA: Use at a concentration of 0.1-1.0ug/mL. For IP : Use at a concentration of 5-10ug/ml. For WB: USe at a concentration of 1-3ug/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
Specificity | Recognizes human SKP1 (p19) protein at 19kD |
Species Cross Reactivity | Human - Not yet tested in other species |
Positive Controls | HeLa cervical carcinoma, Jurkat leukemia T cell and Wi-38 lung broblast cell lysates |
Target | Skp1 |
Form Supplied | Liquid |
Purification | Protein A affinity purified |
Storage Buffer | PBS, pH 7.4 contianing 0.1% sodium azide |
Storage Instruction | Keep as concentrated solution. Store at 4¨¬C short term. For extended storage add 40-50% glycerol, aliquot and store at -20¨¬C or below. Avoid freeze-thaw cycles. |
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