| Catalog Number | GTX23831 |
| Product Name | KAP1 antibody |
| Product Description | Goat polyclonal to KAP1 |
| Synonyms | KRAB-associated protein 1 Nuclear corepressor KAP-1 TIF1-beta Transcription intermediary factor 1-beta TRIM28: tripartite motif-containing 28 |
| Background | The KRAB (Kruppel Associated Box) domain is minimally about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome. These proteins functionally repress transcription via specific interactions with KAP 1 (KRAB-Associated Protein 1). |
| Clonality | Polyclonal |
| Host | Goat |
| Isotype | IgG |
| Immunogen | Synthetic peptide: SSQELSGGPGDGP, corresponding to amino acids 741-753 of KAP 1. |
| Tested Applications | Immunofluorescence, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | WB: Use at an assay dependent dilution. Predicted molecular weight: 100 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Species Cross Reactivity | Cross-reacts with Human and Mouse (see review). Not yet tested in other species. |
| Positive Controls | HepG2 whole cell lysate. |
| Target | KAP1 |
| Form Supplied | Liquid |
| Purification | IgG fraction |
| Purification Note | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Storage Buffer | Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA |
| Storage Instruction | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
Image:img4341.jpgGTX23831 staining (0.5g/ml) of HepG2 lysate (RIPA buffer, 30g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. GTX23831.jpgPrint:Print
