| Catalog Number | GTX82883 |
| Product Name | KAP1 antibody |
| Product Description | Rabbit Polyclonal Antibody to KAP-1 |
| Synonyms | KRAB-Associated Protein 1, Kruppel-Associated Box-Associated Protein 1 |
| Background | The KRAB (Kruppel-associated box) domain is minimally about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome. These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1). KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression. KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression. Recent studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Immunogen | Recombinant human KAP-1 |
| Antigen Species | Human |
| Tested Applications | Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | GTX82883 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~87 kDa protein representing recombinant human KAP-1. This antibody also detects a smaller protein that is believed to be KAP-1 degradation product. Suggested dilution: WB - 1:500. |
| Specificity | GTX82883 detects recombinant human KAP-1 (Kruppel-associated box-associated protein 1). |
| Species Cross Reactivity | Human, others not tested. |
| Target | KAP1 |
| Form Supplied | 100 ug of purified IgG in PBS containing 1 0.05% sodium azide. |
| Storage Instruction | -20º C, Avoid Freeze/Thaw Cycles |
