| Catalog Number | GTX11304 |
| Product Name | alpha Tubulin [B-5-1-2] antibody |
| Product Description | Mouse monoclonal [B-5-1-2] to alpha Tubulin |
| Synonyms | 7277 | TUBA1 | 191110 | P68366 | FLJ30169 | H2-ALPHA | |
| Background | Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin. |
| Clonality | Monoclonal |
| Clone No | B-5-1-2 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Sarkosyl-resistant filaments from Stronglycentrotus purpuratus (sea urchin). |
| Tested Applications | Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | ICC: Use at a concentration of 0.5 - 1 μg/ml, this concentration is recommended using cultured chicken fibroblasts (CFB). IF: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. RIA: Use at an assay dependent dilution. WB: Use at a concentration of 0.25 - 0.5 μg/ml, this concentration is recommended using a total cell extract of the human foreskin fibroblast cell line (FS11). Predicted molecular weight: 50 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Species Cross Reactivity | Cross-reacts with Human, Mouse, Rat, Cow, African Green Monkey, Chicken, Chlamydomonas reinhardtii, kangaroo rat and Sea Urchin. Not yet tested in other species. |
| Positive Controls | Total cell extract of the human foreskin fibroblast cell line (FS11) |
| Target | alpha Tubulin |
| Cellular Localization | Major constituent of microtubules. |
| Form Supplied | Liquid |
| Purification | Protein A purified |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
| Storage Instruction | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Notes | Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use." |
