| Catalog Number | GTX27750 |
| Product Name | alpha Tubulin [TU-01] antibody |
| Product Description | Mouse monoclonal [TU - 01] to alpha Tubulin |
| Synonyms | TUBA1 Tubulin alpha 1 chain Tubulin alpha |
| Background | Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin. |
| Clonality | Monoclonal |
| Clone No | TU-01 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Fraction of tubulin purified from pig brain by two cycles of polymerization-depolymerization. |
| Antigen Species | Pig |
| Tested Applications | ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | ELISA: Use at an assay dependant dilution. ICC: Use at a dilution of 1/100. For ICC, we recommend not only fixing but also permeabilizing the cells. IF: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. GTX27749 (TU-16)is better for IP application. WB: Use at an assay dependant dilution. Detects a band of approximately 55 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Species Cross Reactivity | Cross-reacts with all Mammals and a wide range of other species. Not yet tested in other species. |
| Target | alpha Tubulin |
| Cellular Localization | Major constituent of microtubules. |
| Form Supplied | Liquid |
| Concentration | mg/ml |
| Purification | >95% by SDS-PAGE |
| Purification Note | Purified from ascites using precipitation methods. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
| Storage Instruction | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
