| Catalog Number | GTX75145 |
| Product Name | PCNA Antibody |
| Product Description | Mouse Antibody to PCNA |
| Background | PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. It acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. |
| Clonality | Monoclonal |
| Clone No | PC10 |
| Host | Mouse |
| Isotype | IgG2a (Mouse) |
| Immunogen | Rat PCNA made in the protein A expression vector pR1T2T |
| Antigen Species | Rat |
| Tested Applications | IHC (Frozen sections), IHC (Formalin-fixed paraffin-embedded sections). The usefulness of this product in other applications has not been determined. |
| Application Note | IHC: Suppliead as ready-to-use for IHC. This product does not require protein digestion pre-treatment of paraffin sections. This product requires antigen retrieval using heat pre-treatment prior to staining of paraffin sections. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
| Specificity | Recognises the proliferating cell nuclear antigen (PCNA), a nuclear protein vital for cellular DNA synthesis. |
| Species Cross Reactivity | Rat, Insects & Vertebrates - Not yet tested in other species |
| Positive Controls | Tonsil or colon |
| Target | PCNA |
| Form Supplied | Liquid |
| Purification | Purified IgG |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 1% BSA and 0.09% sodium azide |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Notes | For western blot & immunopresipitation we recommend GTX75143 |
