Catalog Number | GTX21325 |
Product Name | V5 tag antibody (HRP) |
Product Description | Rabbit polyclonal to V5 tag ( HRP ) |
Synonyms | GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST tag V5 epitope tag |
Background | The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). |
Clonality | Polyclonal |
Host | Rabbit |
Isotype | unknown |
Immunogen | Synthetic peptide: GKPIPNPLLGLDST conjugated to KLH. |
Antigen Species | N/A |
Tested Applications | ELISA, Immunocytochemistry, Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | Researchers should determine optimum dilutions for individual applications. |
Specificity | In a direct ELISA against 1 ug of V5 peptide coupled to BSA, conjugate diluted to 1/20,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate. |
Target | V5 tag |
Conjugation | HRP |
Conjuation Note | Conjugated to horseradish peroxidase (HRP). |
Form Supplied | Liquid0.2% BSA |
Purification | Immunogen affinity purified |
Purification Note | Antibodies were immunoaffinity purified using the peptide conjugated to a solid phase support and conjugated to horseradish peroxidase. |
Storage Buffer | 50 mM HEPES pH 7.0, 0.1 M NaCl, 0.2% BSA, 0.01% Thimerosal |
Storage Instruction | Keep as concentrated solution, aliquot and store at 4¡ÆC. |