Catalog Number | GTX23550 |
Product Name | V5 tag antibody (HRP) |
Product Description | Chicken polyclonal to V5 tag ( HRP ) |
Synonyms | GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST tag V5 epitope tag |
Background | The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). |
Clonality | Polyclonal |
Host | Chicken |
Isotype | IgY |
Immunogen | Synthetic peptide: GKPIPNPLLGLDST conjugated to KLH. |
Tested Applications | ELISA, Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | Western blotting: use at a dilution of 1/1000. ELISA: a 1/10000 dilution gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification. Not tested in other applications. Optimal dilutions / concentrations should be determined by the end user. |
Target | V5 tag |
Conjugation | HRP |
Conjuation Note | Conjugated to horseradish peroxidase (HRP). |
Form Supplied | Liquid |
Purification | Immunogen affinity purified |
Storage Buffer | 50 mM HEPES pH 7.0, 0.1 M NaCl, 0.2% BSA, 0.01% Thimerosal |
Storage Instruction | Keep as concentrated solution, aliquot and store at 4¡ÆC. |
Notes | all |