Catalog Number | GTX29137 |
Product Name | V5 tag antibody |
Product Description | Goat polyclonal to V5 tag |
Synonyms | GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST tag V5 epitope tag |
Background | The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). |
Clonality | Polyclonal |
Host | Goat |
Isotype | unknown |
Immunogen | Synthetic peptide: GKPIPNPLLGLDST (V5) conjugated to KLH. |
Tested Applications | ELISA, Immunocytochemistry, Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | ELISA: Use at a dilution of: Coating: 1:100 - 1:500Primary: 1:1000 - 1:30,000. ICC: Use at a dilution of 1/200 - 1/2000. WB: Use at a dilution of 1/1000 - 1/30000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
Specificity | Antibody was verified by ELISA against peptide conjugated to BSA(GKPIPNPLLGLDST/BSA). |
Target | V5 tag |
Form Supplied | Liquid |
Concentration | 1 mg/ml |
Purification | Immunogen affinity purified |
Purification Note | This antibody was affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG. |
Storage Buffer | PBS, pH 7.2, sodium azide, 0.1% |
Storage Instruction | Keep as concentrated solution, aliquot and store at 4¡ÆC. Do not freeze. |