| Catalog Number | GTX40844 |
| Product Name | Vaccinia virus antibody (HRP)*B1G1 |
| Product Description | Rabbit polyclonal to Vaccinia Virus (HRP) |
| Synonyms | Orthopoxvirus A27L |
| Background | Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Immunogen | Lister Strain (mixture of virions and infected cell polypeptides) |
| Tested Applications | ELISA. The usefulness of this product in other applications has not been determined. |
| Application Note | ELISA: Use at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | Recognizes purified virions. Does not cross-react with Parainfluenza (1-3), RSV, Adenovirus, Influenza A & B or HSV-1. Does not react with uninfected cells. Reactive with Lister, Wyeth, New York City and MVA strains of Vaccinia. |
| Species Cross Reactivity | Cross-reacts with Vaccinia Virus. |
| Target | Vaccinia virus |
| Cellular Localization | Whole virus |
| Conjugation | HRP |
| Form Supplied | Liquid |
| Purification | IgG fraction |
| Storage Buffer | Phosphate-buffered saline containing 10mg/ml bovine serum albumin and 0.002% thimerosal |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
