Catalog Number | GTX23346 |
Product Name | VAMP1 antibody |
Product Description | Rabbit polyclonal to VAMP1 |
Synonyms | 25624 | Vamp1 | Syb1 | |
Background | The Vesicle Associated Membrane Proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimers disease, VAMP levels of all isoforms appear to be significantly lowered. It has been shown that the extreme carboxy-terminus of VAMP-1 is involved in subcellular vesicle targeting. |
Clonality | Polyclonal |
Host | Rabbit |
Isotype | IgG |
Immunogen | Synthetic peptide: MSAPAQPPAEGTEGAAPG-C, corresponding to amino acids 1-18 of Rat VAMP1. |
Antigen Species | Rat |
Tested Applications | Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | Western Blot: use at a concentration of 0.3 ¥ìg/ml. Immunoprecipitation: assay dependent. Not tested in other applications. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a 15 kDa protein representing VAMP 1 from rat brain whole protein extract. |
Species Cross Reactivity | Cross-reacts with Human, Rat, Mouse and Dog. Not yet tested in other species. |
Target | VAMP1 |
Form Supplied | Liquid |
Purification | Immunogen affinity purified |
Storage Buffer | PBS, 1 mg/ml BSA, 0.05% sodium azide |
Storage Instruction | Aliquot and store at -20 ¡ÆC long-term. Avoid repeated freeze / thaw cycles. |
Image:img4020.jpg Western blot of VAMP-1 on rat brain extract using GTX23346. Print:Print