Catalog Number | GTX14279 |
Product Name | VAMP2 antibody |
Product Description | Chicken polyclonal to VAMP2 |
Synonyms | 6844 | VAMP2 | 185881 | P63027 | SYB2 | VAMP-2 | Vesicle-associated membrane protein 2 Synaptobrevin-2 |
Background | Synaptobrevins/VAMPs, syntaxins, and the 25 kD synaptosomal associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP2 is a member of the vesicle associated membrane protein (VAMP)/synaptobrevin family. VAMP2 is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. VAMP2 is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. |
Clonality | Polyclonal |
Host | Chicken |
Isotype | IgY |
Immunogen | Synthetic peptide, corresponding to amino acids 43-93 of VAMP2. |
Tested Applications | Immunocytochemistry, Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | For ICC: Use at a dilution of 1:100 - 1:300. For WB: Use at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
Species Cross Reactivity | Human, mouse and rat - Not tested in other species |
Positive Controls | AtT20 cells |
Target | VAMP2 |
Cellular Localization | Type IV membrane protein. Neuronal synaptic vesicles |
Form Supplied | Liquid |
Purification | Affinity purified with antigen |
Storage Buffer | PBS, no preservatives added |
Storage Instruction | Keep as concentrated solution. Store at 4¨¬C short term. For extended storage aliquot and store at -20¨¬C or below. Avoid freeze-thaw cycles. |
Image:img24702.jpgImmunocytochemistry staining of endogenous Vamp2 in AtT20cells. GTX14279 dilution: 1:200. FITC visualisation. Print:Print