| Catalog Number | GTX23347 |
| Product Name | VAMP2 antibody |
| Product Description | Rabbit polyclonal to VAMP2 |
| Synonyms | 24803 | Vamp2 | RATVAMPB | RATVAMPIR | SYB | Syb2 | |
| Background | The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP 25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimers disease, VAMP levels of all isoforms appear to be significantly lowered. It is suggested that VAMP 2 is a resident protein of the insulin-sensitive glucose transporter type 4 (GLUT4) compartment and that it is required for GLUT4 vesicle incorporation into the cell surface in response to insulin. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Immunogen | Synthetic peptide: MSATAATVPPAAPAGEGG-C, corresponding to amino acids 1-18 of Rat VAMP2. |
| Antigen Species | Rat |
| Tested Applications | Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | Western Blot: use at a concentration of 0.3 μg/ml. Immunoprecipitation: assay dependent. Not tested in other applications. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a 19 kDa protein representing VAMP 2 from rat brain whole protein extract. |
| Species Cross Reactivity | Cross-reacts with Rat and Mouse. Not yet tested in other species. |
| Target | VAMP2 |
| Cellular Localization | Type IV membrane protein. Neuronal synaptic vesicles |
| Form Supplied | Liquid |
| Purification | Immunogen affinity purified |
| Storage Buffer | PBS, 1 mg/ml BSA, 0.05% sodium azide |
| Storage Instruction | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
Image:img4021.jpg Western blot of VAMP-2 on rat brain extract using GTX23347. Print:Print
