Catalog Number | GTX28275 |
Product Name | Yersinia pestis [YPF19] antibody |
Product Description | Mouse monoclonal [YPF19] to Yersinia pestis |
Background | This antibody is used to detect Yersinia pestis. Y. pestis has a number of virulence factors that enable it to survive in humans by facilitating use of host nutrients, causing damage to host cells, and subverting phagocytosis and other host defense mechanisms. Capsular F1 antigen is a main immunochemical component of Y. pestis surface. Its synthesis is determined by pFra plasmid and induced at 37oC. F1 is aggregated on the outer membrane of the Y. pestis microbe as an olygomeric protein, forming a granular layer and gradually diffusing into the environment. Capsular polymer consists of multiple similar hydrophobic protein subunits, aggregating at physiological conditions. F1 subunit of the antigen is originally synthesized from 170 amino acids and has a molecular mass 17.6 K, then, after the elimination of the signal peptide during the secretion onto the surface, a protein with molecular mass 15.6 K and isoelectric point 4.1 is formed |
Clonality | Monoclonal |
Clone No | YPF19 |
Host | Mouse |
Isotype | IgG1 |
Immunogen | Purified F1 antigen from Yersinia pestis vaccine strain EV76. |
Tested Applications | ELISA, IHC (Frozen sections), Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | ELISA: Use at an assay dependent dilution. Using double antibody sandwich ELISA with this antibody, the detection limit for the immunoassay is 0.5 ng/ml. IHC-Fr: Use at an assay dependent concentration. WB: Use at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
Specificity | This antibody recognises Y.pestis F1 capsular antigen. There is no cross-reactivity with Y.pseudotuberculosis and Y. enterocolitica. |
Target | Yersinia pestis |
Cellular Localization | Cell Membrane |
Form Supplied | Liquid |
Purification | Protein G purified |
Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.1% sodium azide |
Storage Instruction | Keep as concentrated solution, aliquot and store at 4¡ÆC. Do not freeze. We recommend to parafilm vials during storage to prevent evaporation of buffer. |