| Catalog Number | GTX12869 |
| Product Name | ZAP70 phospho Y315, Y319 antibody |
| Product Description | Rabbit polyclonal to ZAP70 ( phospho Y315 + Y319 ) |
| Synonyms | 7535 | ZAP70 | 176947 | Q6PIA4 | SRK | STD | TZK | ZAP-70 | |
| Background | ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling. Phosphorylation of tyrosines 315 and 319 plays an important role in mediating the positive and negative selection of T cells in thymus. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Immunogen | Synthetic peptide derived from the region of human ZAP70 that contains tyrosines 315 and 319 (unfortunately, the amino acid sequence is considered to be commercially sensitive) |
| Antigen Species | Human |
| Tested Applications | Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | WB: Use at a dilution of 1/1000. Predicted molecular weight: 74.3 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | Syk (85% homologous) may cross react in some cell systems. |
| Species Cross Reactivity | Cross-reacts with Human. Expected to cross-react with Mouse due to sequence homology. Not yet tested in other species. |
| Positive Controls | Jurkat cells treated with hydrogen peroxide |
| Target | ZAP70 |
| Cellular Localization | Cytoplasmic |
| Purification | Immunogen affinity purified |
| Purification Note | The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated ZAP70. The final product is generated by affinity chromatography using a ZAP70-derived peptide that is phosphorylated at tyrosines 315 and 319. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: Dulbeccos PBS without magnesium and calcium, 50% Glycerol, PBS, 1mg/ml BSA, pH 7.3 |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Notes | Centrifuge before opening to settle contents. |
