Catalog Number | GTX18371 |
Product Name | ZAP70 [1E7.2] antibody |
Product Description | Mouse monoclonal [1E7.2] to ZAP70 |
Synonyms | 7535 | ZAP70 | 176947 | Q6PIA4 | SRK | STD | TZK | ZAP-70 | |
Background | ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling. Phosphorylation of tyrosines 315 and 319 plays an important role in mediating the positive and negative selection of T cells in thymus.Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course. Recent studies suggest that protein levels of ZAP70 are elevated in B cells of CLL patients with non mutant heavy chain variable region (IgVH) but not those with the mutant regions. Recent evidence suggests that ZAP70 could be an excellent prognostic biomarker with high levels of the proteins indicating a poor prognosis. |
Clonality | Monoclonal |
Clone No | 1E7.2 |
Host | Mouse |
Isotype | IgG1 |
Immunogen | GST-fusion protein: RRIDTLNSD GYTPEPARIT SPDKPRP, corresponding to amino acids 282-307 of Human ZAP70. |
Antigen Species | Human |
Tested Applications | ELISA, Flow cytometry/FACS, Immunofluorescence, Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | For ELISA: Use at an assay dependent dilution. For FACS: Use at an assay dependent dilution. IF: Use 0.5¥ìg for 10^6 cells in 100ul volume. IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.5-1 ¥ìg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
Species Cross Reactivity | Cross-reacts with Human and Mouse. Not yet tested in other species. |
Positive Controls | Jurkat cell lysate |
Target | ZAP70 |
Cellular Localization | Cytoplasmic |
Purification Note | The antibody was purified by affinity chromatography. |
Storage Buffer | Preservative: 0.09% Sodium Azide. Constituents: PBS. pH 7.2 |
Storage Instruction | Keep as concentrated solution. Store at 4¨¬C short term. For extended storage aliquot and store at -20¨¬C or below. Avoid freeze-thaw cycles. |