| Catalog Number | GTX104373 |
| Product Name | 20S Proteasome alpha1 antibody |
| Full Name | proteasome (prosome, macropain) subunit, alpha type, 1 |
| Product Description | Rabbit polyclonal antibody to 20S Proteasome alpha1 (proteasome (prosome, macropain) subunit, alpha type, 1) |
| Synonyms | HC2 | MGC14542 | MGC14575 | MGC14751 | MGC1667 | MGC21459 | MGC22853 | MGC23915 | NU | PROS30 |
| Background | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Immunogen | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 214 of 20S Proteasome alpha1 |
| Antigen Species | human |
| Tested Applications | WB and IHC-P. The usefulness of this product in other applications has not been determined. |
| Application Note | Suggested starting dilutions are as follows. WB: 1:500-1:3000,IHC-P: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Specificity | This antibody is specific to the human 20S Proteasome alpha1 protein. |
| Species Cross Reactivity | human - Not yet tested in other species |
| Positive Controls | 293T | A431 | H1299 | HeLa | HepG2 | Molt-4 | Raji |
| Target | 20S Proteasome alpha1 |
| Predicted Target Size(KDa) | 30.25 |
| Cellular Localization | Cytoplasm | Nucleus |
| Form Supplied | Liquid |
| Concentration | 1 mg/ml |
| Purification | Purified by antigen-affinity chromatography. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Storage Instruction | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
Image:GTX104373 WB ImageSample (30 ug of whole cell lysate) A: Molt-4 (GTX27912) B: Raji 12% SDS PAGE GTX104373 diluted at 1:1000GTX104373 IHC ImageImmunohistochemical analysis of paraffin-embedded A549 Xenograft, using 20S Proteasome alpha1(GTX104373) antibody at 1:500 dilution.Print:Print
