Catalog Number | GTX77429 |
Product Name | T7 tag antibody |
Product Description | Affinity Purified Rabbit anti - T7 tag |
Clonality | Polyclonal |
Host | Rabbit |
Immunogen | Rabbits were immunized with MASMTGGQQMG (T7 epitope) conjugated to KLH. After multiple immunizations in Freunds adjuvant, serum was collected. Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support |
Antigen Species | Rabbit |
Tested Applications | Western blot. The usefulness of this product in other applications has not been determined. |
Target | T7 tag |
Form Supplied | Liquid |
Storage Buffer | Phosphate Buffered Saline, pH 7.2, 0.1% sodium azide |
Storage Instruction | Keep as concentrated solution, aliquot and store at 4¡ÆC. |
Notes | WB: Recommended dilution range 1:5000 - 1:10,000. For Research and Manufacturing Only; Not for Use in Diagnostic Applications. The affinity purified antibodies were analyzed using ELISA and Western Blot analyses.ELISA: The anti T7 diluted 1:35,000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification. ICL, Inc. HRPO conjugated donkey anti-rabbit IgG was used and TMB was the substrate" |
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