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Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-25-398963 |
GTX72702(R) |
Proliferating Cell Nuclear Antigen (PCNA) (PC10) Antibody//Human.ReactswithallVertebrate,Insects,,Saccharomyc/Mouse |
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Subject
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Proliferating Cell Nuclear Antigen (PCNA) (PC10) Antibody |
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Description
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/ Human.ReactswithallVertebrate,Insects,,Saccharomyc/Mouse |
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Clonality
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Mono |
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Company
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GeneTex |
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Application
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IHC-Fr, IHC-P, IP, Western Blot |
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Conjugation
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Immunogen
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Contents
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| Catalog Number | GTX72702 | | Product Name | Proliferating Cell Nuclear Antigen (PCNA) (PC10) Antibody | | Product Description | Mouse Monoclonal to Proliferating Cell Nuclear Antigen ( PCNA ) | | Background | The 36 kD proliferating cell nuclear antigen was originally defined as an intranuclear polypeptide whose synthesis reaches its maximum during the S phase of the cell cycle.This antibody reacts with proliferating cells in a wide range of normal tissues, but the proportion of proliferating cells with PCNA nuclear immunoreactivity is currently unclear. | | Clonality | Monoclonal | | Clone No | PC10 | | Host | Mouse | | Isotype | IgG2a | | Immunogen | BALB/C mice were immunized with rat PCNA made in the protein A expression vector PR1T2T. | | Antigen Species | Rat | | Tested Applications | IHC (Frozen sections), IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. | | Application Note | Immunohistochemistry (Paraffin/Frozen) 1:50-1:100, . Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 with AutoProbe III (GTX28620). When using routinely processed tissues, a high temperature antigenic unmasking technique is required.( 10 mM citrate, pH 6.0 boiling the tissue for 10-20 mins. followed by cooling to RT for 10-20 mins). This antibody is also suitable for cryostat sections fixed in 4% buffered formalin and ethanol. IP (Native and Denatured) (Using Protein A, antibody at 2 micrograms/ mg protein lysate). WB: 1:100-1:200 for 2 Hrs. at RT. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good diluent to use with most antibodies. | | Species Cross Reactivity | Human. Reacts with all Vertebrate, Insects, and Saccharomyces. | | Positive Controls | Spleen, Tonsil, Carcinomas | | Target | Proliferating Cell Nuclear Antigen ( PCNA ) | | Cellular Localization | Nuclear | | Form Supplied | Dehydrated | | Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. | |
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