| Catalog Number | GTX12427 |
| Product Name | D4 GDI antibody |
| Product Description | Rabbit polyclonal to D4 GDI |
| Synonyms | 397 | ARHGDIB | 602843 | P52566 | D4 | GDIA2 | GDID4 | LYGDI | Ly-GDI | RAP1GN1 | |
| Background | GDP-dissociation inhibitors or GDIs, modulate the activation of GTPases, and may also be involved in the cellular compartmentalization of GTPases. GDIs modulate GTPase activity by binding to small G Proteins, and regulating GDP/GTP exchange. This family of proteins includes Rho-GDI (GDI-1), D4 GDI (GDI-2) and Rho-GDI-3 (GDI-3). Rho-GDI and D4 GDI are both localized in cystol. RhoGDI and GDI/D4 are both members of the Rho subfamily of GTPases. These two proteins are highly homologous, differing primarily in the first 25 amino acids of the N-terminus. D4 GDI has been described as a Caspase 3 (CPP32/YAMA/Apopain) substrate during Fas-induced apoptosis and is located in the cytosol. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Immunogen | Synthetic peptide: KAPEPHVEEDDDDELDSKC conjugated to KLH, corresponding to amino acids 4-21 of Human D4 GDI. |
| Antigen Species | Human |
| Tested Applications | Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | IP: 4 μg is recommended to immunoprecipitate D4 GDI from 500 μg of Jurkat RIPA lysate. WB: Use at a concentration of 0.5 - 1 μg/ml. THis concentration is recommended for blotting using RIPA lysates from human A431, HL-60 and Jurkat cells, anti-rabbit IgG-peroxidase conjugate and a chemiluminescent detection system. Predicted molecular weight: 28 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | Ab12427 specifically reacts with D4 GDI and does not recognize Rho-GDI or GDI-3. |
| Species Cross Reactivity | Expected to cross-react with Human (100% identity with immunogen) and Cow (88% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Positive Controls | RIPA lysates from human A431, HL-60 and Jurkat cells |
| Target | D4 GDI |
| Cellular Localization | Cytoplasmic and Nuclear |
| Purification | Protein A purified |
| Purification Note | The IgG fraction of antiserum is purified using protein A. |
| Storage Buffer | Preservative: 0.035% Sodium Azide; Constituents: 30% Glycerol, 0.105M Sodium Chloride, 0.07M Tris-glycine. pH 7.4 |
| Notes | If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use." |
