Catalog Number | GTX101955 |
Product Name | JAK1 antibody |
Full Name | Janus kinase 1 (a protein tyrosine kinase) |
Product Description | Rabbit polyclonal antibody to JAK1 (Janus kinase 1 (a protein tyrosine kinase)) |
Synonyms | JAK1A | JAK1B | JTK3 |
Background | Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs. [provided by RefSeq] |
Clonality | Polyclonal |
Host | Rabbit |
Immunogen | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 744 and 1037 of JAK1 |
Antigen Species | human |
Tested Applications | WB. The usefulness of this product in other applications has not been determined. |
Application Note | Suggested starting dilutions are as follows. WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
Specificity | This antibody is specific to the human JAK1 protein. |
Species Cross Reactivity | human - Not yet tested in other species |
Positive Controls | A549 | HeLa | HepG2 | HCT116 |
Target | JAK1 |
Predicted Target Size(KDa) | 133.3 |
Cellular Localization | Endomembrane system; Peripheral membrane protein |
Form Supplied | Liquid |
Concentration | 5.94 mg/ml |
Purification | Affinity purified by Protein A. |
Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
Storage Instruction | Keep as concentrated solution. Aliquot and store at -20¨¬C or below. Avoid multiple freeze-thaw cycles. |
Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
Image:GTX101955 WB ImageSample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 5% SDS PAGE GTX101955 diluted at 1:1000 Print:Print