Catalog Number | GTX100604 |
Product Name | JIP-1 antibody |
Full Name | mitogen-activated protein kinase 8 interacting protein 1 |
Product Description | Rabbit polyclonal antibody to JIP-1 (mitogen-activated protein kinase 8 interacting protein 1) |
Synonyms | IB1 | JIP-1 | JIP1 | PRKM8IP |
Background | The protein encoded by this gene is a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes. [provided by RefSeq] |
Clonality | Polyclonal |
Host | Rabbit |
Immunogen | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 112 and 480 of JIP-1 |
Antigen Species | human |
Tested Applications | WB,IHC-P,IF. The usefulness of this product in other applications has not been determined. |
Application Note | Suggested starting dilutions are as follows. WB: 1:500-1:3000,IHC-P: 1:100-1:500,IF:1:100-1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
Specificity | This antibody is specific to the human JIP-1 protein. |
Species Cross Reactivity | human - Not yet tested in other species |
Positive Controls | 293T | A431 | HepG2 | Molt-4 | Raji |
Target | JIP-1 |
Predicted Target Size(KDa) | 77.54 |
Cellular Localization | Cytoplasm (By similarity) | Cytoplasm | perinuclear region (By similarity) | Nucleus (By similarity) |
Form Supplied | Liquid |
Concentration | 1 mg/ml |
Purification | Purified by antigen-affinity chromatography. |
Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
Storage Instruction | Keep as concentrated solution. Aliquot and store at -20¨¬C or below. Avoid multiple freeze-thaw cycles. |
Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
Image:GTX100604 WB ImageSample(30 ug whole cell lysate)A:Hep G2 (GTX27900)7.5% SDS PAGEGTX100604 diluted at 1:1000GTX100604 IF ImageImmunofluorescence analysis of A431, using JIP-1 (GTX100604) antibody at 1:200 dilution.Print:Print