Catalog Number | GTX29113 |
Product Name | V5 tag antibody |
Product Description | Chicken polyclonal to V5 tag |
Synonyms | GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, V5 epitope tag, |
Background | The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). |
Clonality | polyclonal |
Host | Chicken |
Isotype | unknown |
Immunogen | GKPIPNPLLGLDST (V5 epitope) conjugated with KLH. |
Tested Applications | ELISA, Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | Western blotting: 1/5000 |
Specificity | Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GKPIPNPLLGLDST-BSA). A 1:100,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP conjugated goat anti chicken IgY (IgG) and TMB as the substrate. Appropriate specificity controls were run. |
Target | V5 tag |
Form Supplied | Liquid |
Purification | IgY fraction |
Purification Note | Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.allAntibody concentration was determined by extinction coefficient : O.D. 1.32 at 280nM equals 1.0 mg of IgY (IgG). |
Storage Buffer | PBS, pH 7.2, sodium azide, 0.1% |
Storage Instruction | Keep as concentrated solution, aliquot and store at 4¡ÆC. |
Notes | all |