| Catalog Number | GTX28995 |
| Product Name | E Cadherin [5H9] antibody |
| Product Description | Mouse monoclonal [5H9] to E Cadherin |
| Synonyms | Cadherin 1, |
| Background | Cadherins constitute a family of proteins, the members of which are differentially expressed in the different tissues. One of the best characterized members is E-cadherin, which is prevalent in epithelial tissues. It has been shown to play a crucial role in the process of tumor cell metastasis. |
| Clonality | Monoclonal |
| Clone No | 5H9 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Affinity purified 80kD extracellular fragments of E-Cadherin derived from tryptic digestion of A-431 human vulva carcinoma cells |
| Antigen Species | Human |
| Tested Applications | Immunocytochemistry, IHC (Frozen sections), IHC (Formalin-fixed paraffin-embedded sections), Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | ICC: Use at an assay dependent dilution. IHC-P: 1/50 - 1/100. IHC-Fr: 1/50 - 1/100. WB: 1/100 - 1/500. |
| Specificity | Specific reactivity with 120 kDa protein and its 80 kDa trypsin-resistant extracellular part |
| Species Cross Reactivity | Cross-reacts with Human and Mouse. Not yet tested in other species. |
| Target | E Cadherin |
| Form Supplied | Liquid |
| Purification | Protein G affinity purified. |
| Storage Buffer | PBS, 0.09% Sodium Azide |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
