Catalog Number | GTX77460 |
Product Name | E tag antibody |
Product Description | Affinity Purified Rabbit anti E - tag |
Clonality | Polyclonal |
Host | Rabbit |
Immunogen | Rabbits were immunized with peptide conjugated with KLH. After multiple immunizations in Freunds adjuvant, serum was collected. Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase |
Antigen Species | Rabbit |
Tested Applications | Western blot. The usefulness of this product in other applications has not been determined. |
Target | E tag |
Form Supplied | Liquid |
Storage Buffer | PBS, pH 7.2, 0.1% sodium azide |
Storage Instruction | Keep as concentrated solution, aliquot and store at 4¡ÆC. |
Notes | WB: Recommended dilution range 1:1,000 -1:3,000. For Research and Manufacturing Only; Not for Use in Diagnostic Applications. ELISA: Antibody specificity was verified by ELISA against the peptide conjugated to BSA (E-tag-BSA). A 1:10,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using ICL, Inc HRPO conjugated donkey anti rabbit (IgG) at 1:18,000 and TMB as the substrate. Appropriate specificity controls were run." |