| Catalog Number | GTX70166 |
| Product Name | E2F1 [18E10] antibody |
| Product Description | Mouse monoclonal [18E10] antibody to E2F - 1 |
| Synonyms | E2F1, E2F-1 |
| Background | E2F-1-18E10 recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry. |
| Clonality | Monoclonal |
| Clone No | 18E10 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | The region encoding the DNA binding domain (amino acids 244-264) of E2F-1 expressed in E. coli. |
| Tested Applications | Immunofluorescence, Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For IF: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Predicted molecular weight: 47 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
| Species Cross Reactivity | Human - Not yet tested in other species |
| Positive Controls | E2F-1 transfected Soas2 cells |
| Target | E2F1 |
| Cellular Localization | Nuclear |
| Form Supplied | Liquid |
| Purification | Protein G Affinity Purified |
| Purification Note | From hybridoma tissue culture supernatant |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
