Catalog Number | GTX96638 |
Product Name | E2F3 antibody |
Product Description | Mouse polyclonal to E2F3 |
Synonyms | DKFZp686C18211, E2F-3, KIAA0075, MGC104598 |
Background | The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner. Alternative gene splicing is found in the mouse homolog, but has not reported in human yet. |
Clonality | Polyclonal |
Host | Mouse |
Immunogen | E2F3 (AAH16847, 1 a.a. ~ 134 a.a) full length recombinant protein with GST tag. |
Antigen Species | Human |
Tested Applications | ELISA, Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | For ELISA (tested with immunogen): Use at an assay dependednt dilution. For WB: (tested against immunogen): Use at an assay dependednt dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
Specificity | Not tested |
Species Cross Reactivity | Human - Not yet tested in other species |
Positive Controls | Recombinant protein |
Target | E2F3 |
Form Supplied | Liquid |
Purification | Unpurified |
Purification Note | From polyclonal serum |
Storage Buffer | Antiserum containing 50% glycerol |
Storage Instruction | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20¨¬C or below. Avoid freeze-thaw cycles. |
Notes | Antibody reactive against recombinant protein with GST tag on ELISA. Please enquire about availability of recombinant protein. |
Manufacturer & Mfg Part# | Abnova H00001871-A01Only available to US end-users. Not available outside of the US or for resale inside of the US. |