Catalog Number | GTX108357 |
Product Name | DAP12 antibody |
Full Name | TYRO protein tyrosine kinase binding protein |
Product Description | Rabbit polyclonal antibody to DAP12 (TYRO protein tyrosine kinase binding protein) |
Synonyms | DAP12 | KARAP | PLOSL |
Background | This gene encodes a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL. Two alternative transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described, but their full-length nature has not been deterimined. [provided by RefSeq] |
Clonality | Polyclonal |
Host | Rabbit |
Immunogen | Synthetic peptide contain a sequence corresponding to a region within amino acids 50 and 113 of DAP12 |
Antigen Species | human |
Tested Applications | WB. The usefulness of this product in other applications has not been determined. |
Application Note | Suggested starting dilutions are as follows. WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
Specificity | This antibody is specific to the human DAP12 protein. |
Species Cross Reactivity | human - Not yet tested in other species |
Positive Controls | 293T |
Target | DAP12 |
Predicted Target Size(KDa) | 12.18 |
Cellular Localization | Membrane; Single-pass type I membrane protein |
Form Supplied | Liquid |
Concentration | 0.65 mg/ml |
Purification | Purified by antigen-affinity chromatography. |
Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
Storage Instruction | Keep as concentrated solution. Aliquot and store at -20¨¬C or below. Avoid multiple freeze-thaw cycles. |
Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
Image:GTX108357 WB ImageWestern Blot analysis of TYROBP expression in transfected 293T cell line by TYROBP polyclonal antibody. A: Non-transfected lysate. B: TYROBP transfected lysate. 15% SDS PAGEGTX108357 diluted at 1:500Print:Print