Catalog Number | GTX101235 |
Product Name | E2F1 antibody |
Full Name | E2F transcription factor 1 |
Product Description | Rabbit polyclonal antibody to E2F1 (E2F transcription factor 1) |
Synonyms | E2F-1 | RBAP1 | RBBP3 | RBP3 |
Background | The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq] |
Clonality | Polyclonal |
Host | Rabbit |
Immunogen | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 43 and 187 of Human E2F1 |
Antigen Species | Human |
Tested Applications | WB. The usefulness of this product in other applications has not been determined. |
Application Note | Suggested starting dilutions are as follows. WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
Specificity | This antibody is specific to the Human E2F1 protein. |
Species Cross Reactivity | Human - Not yet tested in other species |
Positive Controls | 293T | A431 | H1299 | HeLaS3 | HepG2 | Molt-4 | Raji |
Target | E2F1 |
Predicted Target Size(KDa) | 46.93 |
Cellular Localization | Nucleus |
Form Supplied | Liquid |
Concentration | 1 mg/ml |
Purification | Purified by antigen-affinity chromatography. |
Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
Storage Instruction | Keep as concentrated solution. Aliquot and store at -20¨¬C or below. Avoid multiple freeze-thaw cycles. |
Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
Image:GTX101235 WB ImageSample (30 ug of whole cell lysate)A: A431 (GTX27909)10% SDS PAGEGTX101235 diluted at 1:500Print:Print