| Catalog Number | GTX105292 |
| Product Name | G protein alpha inhibitor 1 antibody |
| Full Name | guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 |
| Product Description | Rabbit polyclonal antibody to GNAI1 (guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1) |
| Synonyms | Gi |
| Background | Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs (MIM 139320) and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain (GNAO1; MIM 139311); and transducin-1 (GNAT1; MIM 139330) and transducin-2 (GNAT2; MIM 139340), proteins involved in phototransduction in retinal rods and cones, respectively (Sullivan et al., 1986 [PubMed 3092218]; Bray et al., 1987 [PubMed 3110783]). Suki et al. (1987) [PubMed 2440724] concluded that the human genome contains at least 3 nonallelic genes for alpha-i-type subunits of G protein; see, e.g, GNAI2 (MIM 139360), GNAI3 (MIM 139370), and GNAIH (MIM 139180).[supplied by OMIM] |
| Clonality | Polyclonal |
| Host | Rabbit |
| Immunogen | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 23 and 329 of Human GNAI1 |
| Antigen Species | Human |
| Tested Applications | WB. The usefulness of this product in other applications has not been determined. |
| Application Note | Suggested starting dilutions are as follows. WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Specificity | This antibody is specific to the Human GNAI1 protein. |
| Species Cross Reactivity | Human - Not yet tested in other species |
| Positive Controls | 293T | A431 | H1299 | HeLaS3 | Molt-4 |
| Target | GNAI1 |
| Predicted Target Size(KDa) | 40.37 |
| Form Supplied | Liquid |
| Concentration | 1 mg/ml |
| Purification | Purified by antigen-affinity chromatography. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Storage Instruction | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
Image:GTX105292 WB ImageSample(30 ug whole cell lysate)A: A431(GTX27909)B: HeLa S3(GTX14654)12% SDS PAGEGTX105292 diluted at 1:500Print:Print
