Catalog Number | GTX101177 |
Product Name | HADHA antibody |
Full Name | hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit |
Product Description | Rabbit polyclonal antibody to HADHA (hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit) |
Synonyms | ECHA | GBP | HADH | LCHAD | MGC1728 | MTPA | TP-ALPHA |
Background | This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq] |
Clonality | Polyclonal |
Host | Rabbit |
Immunogen | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 459 and 761 of HADHA |
Antigen Species | Human |
Tested Applications | WB and IF. The usefulness of this product in other applications has not been determined. |
Application Note | Suggested starting dilutions are as follows. WB: 1:500-1:3000£¬IF:1:100-1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
Specificity | This antibody is specific to the Human HADHA protein. |
Species Cross Reactivity | Human - Not yet tested in other species |
Positive Controls | 293T | A431 | H1299 | HeLa | HepG2 | Molt-4 | Raji |
Target | HADHA |
Predicted Target Size(KDa) | 83.01 |
Cellular Localization | Mitochondrion |
Form Supplied | Liquid |
Concentration | 1 mg/ml |
Purification | Purified by antigen-affinity chromatography. |
Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
Storage Instruction | Keep as concentrated solution. Aliquot and store at -20¨¬C or below. Avoid multiple freeze-thaw cycles. |
Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
Image:GTX101177 WB ImageSample(30 ¥ìg of whole cell lysate)A:Molt-4 (GTX27912)B:Raji(GTX27908)7.5% SDS PAGEGTX101177 diluted at 1:1500GTX101177 IF ImageImmunofluorescence analysis of HeLa, using HADHA(GTX101177) antibody at 1:200 dilution.Print:Print