| Synonyms | YWHA, FocusOn116 |
| Immunogen | 14.3.3 gamma recombinant protein of human origin |
| Product Type | Antibodies |
| Recommended Isotype Control | SM10P (for use in human samples), AM03095PU-N |
| Storage | Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Laronga C, Yang HY, Neal C, Lee MH. J Biol. Chem 2000. 2. Baldin V. (2000) Prog cell cycle res. 4: 49-60 3. Skoulakis EM, Davis RL. (1998) Mol Neurobiol 3: 269-284. 4. Roberts MR. (2000) Curr Opin Plant Biol 5: 400-405. |
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| Format | State: Liquid purifed Ig fraction |
| ? | Purification: Protein G chromatography |
| ? | Buffer System: 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide |
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| Specificity | This antibody recognizes beta, gamma, sigma and zeta isoforms of 14.3.3. |
| Add. information | Molecular Weight of Antigen: 26-33 kDa Cellular localization: Cytoplasmic |
| Application | Immunoprecipitation (denatured verified): use Protein G, antibody at 2 ?g/mg protein lysate. Western Blotting: 1-2 ?g/ml for 2 hrs at RT. Recommended positive control: HeLa cells. |
| Background | 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. |
| Concentration | 0.2 mg/ml |
| Focuson | 116 Antibody Panel to Proteins of the 14-3-3 family (14-3-3 beta, 14-3-3 gamma, 14-3-3 epsilon, 14-3-3 zeta, 14-3-3 theta, 14-3-3 sigma, 14-3-3 eta) |
