| Synonyms | YWHAZ, KCIP-1, FocusOn116 |
| Swiss Prot Num | P63104 |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human 14-3-3 ?¶ around the phosphorylation site of serine 58 (R-S-Sp-W-R). |
| Product Type | Antibodies |
| Storage | Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Gu YM, et al. (2006) FEBS Lett ; 580(1): 305-310. 2. Powell DW, et al. (2003) Mol Cell Biol; 23(15): 5376-5387. 3. Mackintosh C. (2004) Biochem J; 381(Pt 2): 329-342. |
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| Format | State: Liquid purified Ig fraction. |
| ? | Purification: Immunoaffinity chromatography using epitope-specific phosphopeptide. |
| ? | Buffer System: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. |
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| Specificity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. 14-3-3 ?¶ (Phospho-Ser58) antibody detects endogenous levels of 14-3-3 ?¶ only when phosphorylated at serine 58. |
| Application | Suitable for use in Suitable for use in Western blot (1:500-1:1000) and Immunohistochemistry on paraffin-embedded sections (1:50-1:100). |
| Concentration | 1.0 mg/ml |
| Focuson | 116 Antibody Panel to Proteins of the 14-3-3 family (14-3-3 beta, 14-3-3 gamma, 14-3-3 epsilon, 14-3-3 zeta, 14-3-3 theta, 14-3-3 sigma, 14-3-3 eta) |
